Qhov feem ntau ua rau HPLC ua tsis tiav thiab kev daws teeb meem

Raws li cov cuab yeej ua haujlwm siab, HPLC tuaj yeem yooj yim ua rau qee qhov teeb meem me me yog tias nws tsis ua haujlwm raws li qhov tseeb thaum siv.Ib qho teeb meem feem ntau yog qhov teeb meem ntawm kab compression.Yuav ua li cas sai sai teeb meem ib tug faulty chromatograph.Lub HPLC system feem ntau muaj lub raj mis reservoir, lub twj tso kua mis, lub twj tso kua mis, lub tshuab nqus tsev, ib kem, chav ntsuas kub, lub ntsuas ntsuas thiab cov ntaub ntawv ua haujlwm.Rau tag nrho cov kab ke, cov ncej, cov twj tso kua mis thiab cov cuab yeej ntes yog cov khoom tseem ceeb thiab cov chaw tseem ceeb uas muaj teeb meem.

Tus yuam sij rau kem siab yog thaj chaw uas xav tau kev saib xyuas zoo thaum siv HPLC.Kev ruaj ntseg ntawm sab siab yog ze ze rau chromatographic ncov zoo, kem efficiency, sib cais efficiency thiab tuav lub sij hawm.Kem siab ruaj khov tsis txhais hais tias tus nqi siab ruaj khov ntawm tus nqi ruaj khov, tab sis qhov kev hloov pauv siab yog nyob nruab nrab ntawm 345kPa lossis 50PSI (tso cai rau kev siv cov gradient elution thaum lub siab ruaj khov thiab maj mam hloov).Kev siab dhau los yog qis dhau yog qhov teeb meem ntawm kab.

Qhov feem ntau ua rau HPLC ua tsis tiav thiab kev daws teeb meem

1, kev kub siab yog qhov teeb meem feem ntau hauv kev siv HPLC.Qhov no txhais tau tias muaj kev nce siab sai sai.Feem ntau, muaj cov laj thawj hauv qab no: (1) Feem ntau, qhov no yog vim qhov ntws channel thaiv.Hauv qhov no, peb yuav tsum tshawb xyuas nws ua ntu zus.ib.Ua ntej, txiav tawm qhov inlet ntawm lub tshuab nqus tsev twj.Nyob rau lub sijhawm no, lub raj PEEK tau ntim cov kua kom lub raj PEEK me dua lub raj mis hnyav kom pom tias cov kua ntws los ntawm qhov xav tau.Yog tias cov kua tsis ntws los yog maj mam poob, cov kuab tshuaj lim lub taub hau raug thaiv.Kev kho mob: So hauv 30% nitric acid rau ib nrab ib teev thiab yaug nrog dej ultrapure.Yog hais tias cov kua drips random, cov kuab tshuaj lim lub taub hau yog qhov qub thiab raug kuaj xyuas;b.Qhib lub Purge valve kom lub sijhawm txawb tsis dhau los ntawm kab, thiab yog tias lub siab tsis txo qis, lub taub hau lim dawb raug thaiv.Kev kho mob: Lim whiteheads raug tshem tawm thiab sonicated nrog 10% isopropanol rau ib nrab ib teev.Piv txwv tias lub siab poob qis dua 100PSI, lub taub hau dawb lim yog qhov qub thiab raug kuaj xyuas;c.Tshem tawm qhov kawg ntawm kab ntawv, yog tias lub siab tsis txo qis, cov kem raug thaiv.Kev Kho Mob: Yog tias nws yog qhov tsis muaj ntsev thaiv, yaug 95% kom txog thaum lub siab zoo li qub.Yog tias qhov kev cuam tshuam yog tshwm sim los ntawm qee cov ntaub ntawv khaws cia ntau dua, qhov muaj zog txaus dua li lub sijhawm tam sim no ntawm lub xov tooj ntawm tes yuav tsum tau siv kom maj nrawm mus rau qhov qub.Yog tias lub sij hawm tu lub sij hawm ntev tsis txo qis raws li cov txheej txheem saum toj no, lub inlet thiab qhov hluav taws xob ntawm kem tuaj yeem txiav txim siab tias yuav tsum tau txuas nrog lub ntsuas ntsuas ntawm qhov tsis sib xws, thiab kem tuaj yeem ntxuav nrog lub sijhawm txawb.Nyob rau lub sijhawm no, yog tias lub siab ntawm kem tseem tsis tau txo qis, cov kem nkag sieve phaj tsuas tuaj yeem hloov tau, tab sis ib zaug kev ua haujlwm tsis zoo, nws yog ib qho yooj yim uas yuav ua rau txo qis ntawm cov nyhuv, yog li sim siv tsawg dua.Rau cov teeb meem nyuaj, kev hloov kab yuav raug txiav txim siab.

(2) Kev teeb tsa tus nqi ntws tsis raug: Qhov tseeb txaus tuaj yeem rov pib dua.

(3) Qhov sib piv tsis raug: qhov viscosity Performance index ntawm qhov sib txawv ntawm cov dej ntws sib txawv, thiab qhov sib xyaw ua ke ntawm cov dej ntws nrog cov viscosity siab dua kuj loj dua.Yog tias ua tau, cov kuab tshuaj viscosity qis tuaj yeem hloov lossis rov teeb thiab npaj.

(4) Qhov system siab xoom drift: kho qhov xoom ntawm cov kua theem sensor.

2, lub siab tsis tshua muaj siab (1) feem ntau yog tshwm sim los ntawm qhov system to.Yuav ua li cas: Nrhiav txhua qhov kev sib txuas, tshwj xeeb tshaj yog qhov sib txuas ntawm ob qho kawg ntawm kab, thiab ua kom nruj rau qhov chaw xau.Tshem cov ncej thiab nruj lossis kab PTFE zaj duab xis nrog lub zog tsim nyog.

(2) Cov roj nkag mus rau hauv lub twj tso kua mis, tab sis lub siab feem ntau tsis ruaj khov rau lub sijhawm no, siab thiab qis.Qhov tseeb dua, lub twj tso kua mis yuav tsis tuaj yeem nqus cov kua dej.Txoj kev kho mob: qhib lub valve thiab ntxuav ntawm tus nqi ntws ntawm 3 ~ 5ml / min.Yog tsis yog, huab cua npuas tau aspirated ntawm lub tso pa valve siv ib tug tshwj xeeb koob raj.

(3) Tsis muaj lub sijhawm tawm ntawm lub xov tooj ntawm tes: xyuas seb puas muaj lub sijhawm txawb hauv lub raj xa dej, seb lub dab dej puas nyob rau hauv lub sijhawm txawb, thiab seb lub twj tso kua mis puas khiav.

(4) Lub valve siv tsis raug kaw: qhov siv valve raug kaw tom qab deceleration.Nws feem ntau mus rau 0.1.~ 0.2mL / min tom qab kaw qhov siv valve.

Cov ntsiab lus:

Hauv daim ntawv no, tsuas yog cov teeb meem tshwm sim hauv cov kua chromatography raug tshuaj xyuas.Tau kawg, hauv peb daim ntawv thov, peb yuav ntsib ntau yam teeb meem.Hauv kev ua yuam kev, peb yuav tsum ua raws li cov hauv qab no: tsuas yog hloov ib qho ntawm ib lub sijhawm los txiav txim siab txog kev sib raug zoo ntawm qhov kev xav thiab qhov teeb meem;Feem ntau, thaum hloov qhov chaw rau kev daws teeb meem, peb yuav tsum tau ua tib zoo muab cov khoom tshem tawm tsis zoo rov qab rau hauv qhov chaw kom tsis txhob pov tseg;Tsim ib tus cwj pwm zoo yog tus yuam sij rau kev ua tiav ntawm kev ua txhaum.Hauv kev xaus, thaum siv HPLC, nws yog ib qho tseem ceeb uas yuav tsum tau ua tib zoo saib xyuas cov qauv ua ntej thiab kev ua haujlwm zoo thiab kev saib xyuas cov khoom siv.