Phosphorylation cuam tshuam rau txhua yam ntawm lub neej ntawm tes, thiab cov protein kinases cuam tshuam rau txhua yam ntawm kev sib txuas lus hauv cellular los ntawm kev tswj cov kev taw qhia thiab cov txheej txheem ntawm tes.Txawm li cas los xij, aberrant phosphorylation kuj yog qhov ua rau muaj ntau yam kab mob;Hauv particular, mutated protein kinases thiab phosphatases tuaj yeem ua rau ntau yam kab mob, thiab ntau cov co toxins thiab cov kab mob kuj muaj kev cuam tshuam los ntawm kev hloov cov txheej txheem phosphorylation ntawm intracellular proteins.
Phosphorylation ntawm serine (Ser), threonine (Thr), thiab tyrosine (Tyr) yog cov txheej txheem hloov pauv cov protein.Lawv koom nrog kev tswj hwm ntawm ntau lub cellular kev ua ub no, xws li receptor signaling, protein koom haum thiab segmentation, ua kom los yog inhibition ntawm cov protein muaj nuj nqi, thiab txawm cell ciaj sia taus.Phosphates raug nqi tsis zoo (ob tus nqi tsis zoo rau ib pawg phosphate).Yog li ntawd, lawv ntxiv hloov cov khoom ntawm cov protein, uas feem ntau yog kev hloov pauv, ua rau muaj kev hloov pauv ntawm cov qauv ntawm cov protein.Thaum cov phosphate pab pawg raug tshem tawm, kev sib haum xeeb ntawm cov protein yuav rov qab mus rau nws qhov qub.Yog hais tias ob qho kev sib txuas ntawm cov proteins pom cov dej num sib txawv, phosphorylation tuaj yeem ua qhov hloov pauv rau cov protein kom tswj nws cov haujlwm.
Ntau cov tshuaj hormones tswj cov haujlwm ntawm cov enzymes tshwj xeeb los ntawm kev ua kom lub xeev phosphorylation ntawm serine (Ser) lossis threonine (Thr) residues, thiab tyrosine (Tyr) phosphorylation tuaj yeem tshwm sim los ntawm kev loj hlob (xws li insulin).Cov phosphate pawg ntawm cov amino acids no tuaj yeem raug tshem tawm sai.Yog li, Ser, Thr, thiab Tyr ua haujlwm raws li cov hloov pauv molecular hauv kev tswj hwm ntawm tes ntawm tes xws li qog hlav.
Synthetic peptides ua lub luag haujlwm tseem ceeb hauv kev kawm txog cov protein kinase substrates thiab kev sib cuam tshuam.Txawm li cas los xij, muaj qee yam uas cuam tshuam lossis txwv kev hloov pauv ntawm phosphopeptide synthesis thev naus laus zis, xws li tsis muaj peev xwm ua tiav tag nrho automation ntawm solid-thesis synthesis thiab tsis muaj kev sib txuas yooj yim nrog cov qauv tshuaj ntsuam xyuas.
Lub platform raws li peptide synthesis thiab phosphorylation hloov kho tshuab overcomes cov kev txwv no thaum txhim kho synthesis efficiency thiab scalability, thiab lub platform yog zoo suited rau kev kawm ntawm protein kinase substrates, antigens, khi molecules, thiab inhibitors.
Post lub sij hawm: May-31-2023