Cov tshuaj Peptide feem ntau txhais tau tias yog cov polymers uas muaj amide bonds nrog tsawg dua 40 amino acid residues.Vim muaj kev ua haujlwm siab receptor thiab kev xaiv ntawm cov tshuaj peptide uas tsis tshua muaj kev pheej hmoo ntawm kev phiv, muaj kev txaus siab rau peptides los ntawm kev lag luam kws tshuaj.Thaum lub sijhawm no, kuj tseem muaj ntau lub hnub qub tshuaj, uas feem ntau yog tsom rau hauv kev lag luam kab mob metabolic, xws li GLP-1 analogue somalutide, gastric inhibitory peptide (GIP) glucagon-zoo li peptide-1 (GLP-1) tesiparatide thiab lwm yam dual. - receptor agonists.Tsis tas li ntawd, nrog kev nce ntawm PDC thiab RDC tshuaj.Tam sim no, txoj kev npaj ntawm cov tshuaj polypeptide feem ntau suav nrog tshuaj synthesis thiab lom fermentation.Biofermentation feem ntau yog siv los tsim cov peptides ntev.Qhov zoo yog cov nqi tsim khoom tsawg, tab sis qhov tsis muaj peev xwm ua kom paub cov amino acids tsis zoo rau hauv peptide ib ntus thiab tsis muaj peev xwm ua tau ntau yam kev kho kom zoo nkauj ntawm cov saw peptide.Yog li ntawd, nws daim ntawv thov kuj tseem txwv heev.Cov txheej txheem tshuaj synthesis muaj xws li cov theem khoom sib txuas thiab ua kua theem synthesis.Solid-theem synthesis muaj qhov zoo dua ntawm cov kua-theem synthesis: cov khoom siv ntau dhau tuaj yeem siv rau cov tshuaj tiv thaiv kom ua tiav kev sib txuas.Tshaj cov amino acids, shrinkage agents, thiab by-products tuaj yeem raug tshem tawm los ntawm kev ua haujlwm yooj yim, tsis txhob ua haujlwm tom qab kev ua haujlwm thiab kev ua kom huv thiab txhim kho kev ua haujlwm, yog li cov txheej txheem sib xyaw ua ke tau siv dav tshaj plaws."Chemical synthesis raw cov ntaub ntawv rau synthesis ntawm peptides muaj xws li pib cov ntaub ntawv, reagents, thiab cov kuab tshuaj."Lawv qhov zoo, tshwj xeeb tshaj yog qhov zoo ntawm cov khoom siv thawj zaug, tuaj yeem cuam tshuam rau qhov zoo ntawm API.Cov khoom pib feem ntau yog hais txog cov amino acid derivatives uas tau lees paub rau peptide saw hloov cov roj fatty acids, polyethylene glycol, thiab lwm yam. Raws li cov khoom siv tseem ceeb, lawv raug cais raws li cov ntaub ntawv hauv API qauv, uas yog ncaj qha ntsig txog qhov zoo ntawm API.Yog li ntawd, peb yuav tsum tsom rau kev tswj cov khoom pib.
I. Rationalize thawj cov khoom xaiv
ICHQ11 qhia meej meej tias yog tias cov khoom siv tshuaj lom neeg muag hauv khw yog siv los ua cov khoom siv thawj zaug, tus neeg thov kev pabcuam tsis tas yuav tham txog nws qhov tsim nyog.Cov khoom siv tshuaj uas muag hauv khw feem ntau tuaj yeem siv tsis tau tsuas yog pib cov khoom siv tshuaj, tab sis kuj tuaj yeem muag hauv cov khw muag tshuaj uas tsis yog tshuaj.Customized thiab synthesized compounds tsis yog cov khoom siv tshuaj uas muag hauv khw.Txawm hais tias tsis muaj kev lag luam uas tsis yog tshuaj los tiv thaiv cov amino acids kom tau raws li ICHQ11 cov ntsiab lus ntawm cov tshuaj muag hauv khw, lawv yog cov compact, chemically txawv thiab structurally clear, yooj yim rau cais thiab purify, thiab muaj peev xwm txheeb xyuas thiab ntsuam xyuas los ntawm cov kev analytical txoj kev. .Lawv muaj cov tshuaj lom neeg ruaj khov thiab yooj yim rau khaws cia, thauj, thiab ua ke
Ii.Tswj cov khoom muaj feem xyuam hauv cov khoom pib
Cov lus hais txog kev tiv thaiv amino acids tau muab tso rau hauv API qauv ua ib feem tseem ceeb ntawm cov qauv, uas yog ncaj qha ntsig txog qhov zoo ntawm API.Yog li ntawd, peb yuav tsum nruj me ntsis tswj cov impurity cov ntsiab lus nyob rau hauv thawj cov ntaub ntawv, nkag siab txog kev hloov thiab tshem tawm cov impurities nyob rau hauv cov txheej txheem tsim, thiab thaum kawg qhia meej txog kev sib raug zoo ntawm lawv thiab impurities hauv API.
Nkag siab txog cov khoom siv tshuaj pib tshuaj polypeptide
Thib peb, cov kuab tshuaj residue hauv cov khoom pib
Feem ntau, muab qhov tshwj xeeb ntawm cov theem tsim ntawm peptides, ntau cov kuab tshuaj yuav raug siv los ntxuav cov peptide resin tom qab ua tiav txhua kauj ruam ntawm cov amino acid coupling thiab detachment los ntawm kev tiv thaiv.Crude peptides tau los ntawm kev tawg cov peptide resin kuj yuav ua los ntawm HPLC thiab khov-qhuav.Yog li, tsis tshua muaj kev pheej hmoo tias qhov me me ntawm cov kuab tshuaj txuas nrog cov amino acids tiv thaiv yuav raug xa mus rau API kawg.Txawm li cas los xij, kev saib xyuas tshwj xeeb yuav tsum tau them rau cov seem ntawm acetate, butyl acetate, thiab cov kuab tshuaj cawv, vim cov kuab tshuaj no tuaj yeem ua rau muaj kev phiv nrog cov amino acids lossis peptide chains thaum lub sij hawm sib txuas ntawm cov amino acids.Piv txwv li, thaum lub sij hawm amino acid coupling, cov residual acetic acid yuav hnov mob nrog cov amino pawg ntawm cov peptide saw, uas ua rau lub kaw kawg ntawm peptide saw;Thaum lub sijhawm ua haujlwm ntawm cov amino acid, cov kuab tshuaj residual cawv yuav hnov mob nrog cov active carboxyl pawg, ua rau passivation ntawm active amino acid, txo qhov sib npaug ntawm cov amino acid, thiab thaum kawg ua rau tsis tiav amino acid coupling thiab tsis muaj peptide impurities.Lub tuam txhab tswj hwm butyl acetate, cawv, methanol, thiab acetic acid hauv COA, noj cov amino acid los ntawm Zheng Yuan Biochemical ua piv txwv.Tus qauv rau butyl acetate yog ≤0.5% butyl acetate, uas tau kuaj pom tias yog 0.10%.Raws li ICHQ3C, butyl acetate rau peb hom kuab tshuaj, teeb tus qauv rau 0.5% lossis tsawg dua raws li cov kev cai ntawm ICHQ3C, tab sis xav txog butyl acetate amino acetylation tuaj yeem ua rau muaj kev pheej hmoo, tseem cuam tshuam nrog butyl acetate rau kev tshawb fawb tus qauv. , los txiav txim seb tus qauv tsim nyog dua.
Post lub sij hawm: Aug-29-2023