Nws ua lub luag haujlwm tseem ceeb hauv kev ua neej nyob, phosphorylating Tyrr, Ser, Thr, nyob ntawm peptides.Cov phosphorylated amino acid feem ntau yog siv los tsim cov phosphoric acid peptide, tam sim no siv rau hauv ib qho phenyl phosphorylated amino acids.HBTU / phosphorylated amino acids feem ntau yog siv los txuas cov phosphorylated amino acids HOBt / DIEA txoj kev, tab sis cov phosphoric acid peptide yog xaiv qhov kev tsis zoo no, tshwj xeeb tshaj yog nyob rau hauv lub generated ntau ntev peptide peptides los yog amino acid phosphate, txuas cov efficiency yog tsawg, tsawg. purity ntawm cov khoom kawg.Rau qhov no phosphopeptide, peb xav txog kev xaiv tom qab phosphorylation mus kom ze.Cov txheej txheem tiam yog xaiv tshem tawm cov amino acid sab-chain tiv thaiv pawg uas yuav tsum tau sau npe tom qab peptide synthesis.Rau Tyr, Thr tuaj yeem cuam tshuam ncaj qha siv cov amino acids uas tsis muaj kev tiv thaiv sab chains.-Ser (trt), quantitatively tshem tawm ntawm 1% TFA / DCM ib puag ncig.
Phosphorylation, nrog ob npaug benzene phosphite phosphoramide thiab plaub nitrogen yog tsim thiab phosphoramide tetrazolium active intermediates, txuas nrog hydroxy, ces nyob rau hauv cov kua qaub peroxide oxidation phosphoryl tsim, ua tiav cov tshuaj tiv thaiv.
Tam sim no, txoj kev hloov pauv peptide phosphorylated muaj ob lub ntsiab:
(1) kev taw qhia ncaj qha ntawm nruab nrab tau lees paub phosphorylated amino acids rau hauv cov txheej txheem polypeptide;
(2) tom qab cov resin generated ntawm peptide ib ntus, rau Ser, Tyr, los yog Thr phosphorylation ntawm sab saw hydroxyl.
1) yuav nruab nrab lav phosphorylated amino acids ncaj qha qhia peptide ib ntus:
Ntawd yog, cov amino acids uas yuav tsum tau phosphorylated yuav phosphorylated thiab tsim nyog tiv thaiv ua ntej siv (Thr, Ser lossis Tyr), thiab tom qab ntawd cov phosphorylation yuav txo qis mus rau qhov chaw xaiv ntawm polypeptide Performance index raws li SPPS tiam txheej txheem. .Txoj kev no yooj yim rau kev khiav lag luam, tau dhau los ua txoj hauv kev tseem ceeb ntawm peptide kev lag luam phosphorylated hloov kho.
2) tom qab cov resin generated ntawm peptide ib ntus, rau Ser hydroxy phosphorylation, Tyr, los yog Thr sab chains:
Phosphorylation kho kom zoo nkauj siv cov phosphorylation ntawm ib qho kev cog lus rau hauv txoj kev polypeptide, vim yog cov steric hindrance generated los ntawm cov pab pawg loj nyob rau hauv lub sab saw kho kom zoo nkauj, nws yog ib qho nyuaj rau lub phosphorylation ntawm amino acids ntsws nrog cov peptide saw.Nyob rau hauv lub neej yav tom ntej, kev taw qhia ntawm cov amino acids yuav nyuaj dua, tshwj xeeb tshaj yog nyob rau hauv kev kho kom zoo nkauj uas muaj ntau qhov chaw phosphorylation, tiam yuav dhau los ua nyuaj heev, qhov kawg ntawm cov khoom muaj pes tsawg leeg yog complex, nyuaj rau cais, thiab cov tawm los yog tsawg heev.
Yog li ntawd, thaum ntau qhov chaw hauv cov saw peptide yog phosphorylated, ib tus tuaj yeem xaiv los tsim cov peptide sib lawv liag ntawm cov cob thiab tom qab ntawd phosphorylate nws los ntawm sab saw hydroxyl pawg Ser, Tyr, lossis Thr: nws tiam neeg feem ntau koom nrog kev xaiv tshem tawm. sab saw tiv thaiv pawg uas yuav tsum tau paub txog cov amino acids tom qab peptide synthesis.Rau Tyr, Thr tuaj yeem siv ncaj qha rau cov amino acid sab chains tsis tiv thaiv teb.
Sab saw tiv thaiv puag 1% TFA / DCM ib puag ncig tuaj yeem tshem tawm ntau.Thaum xaiv txoj kev no, ib qho phosphoramidite tetrazolium active intermediate tuaj yeem tsim siv dibenzyl phosphoramidite thiab tetrazolium, txuas mus rau ib pawg hydroxyl, thiab tom qab ntawd oxidized los tsim ib pab pawg phosphoryl hauv peroxyacid ib puag ncig kom ua tiav cov tshuaj tiv thaiv.
Post lub sij hawm: Sep-08-2023