Recombinant protein antigens feem ntau muaj ntau qhov sib txawv ntawm cov epitopes, qee qhov yog cov kab ke epitopes thiab qee qhov yog cov txheej txheem epitopes.Polyclonal antibodies tau txais los ntawm kev txhaj tshuaj tiv thaiv tsiaj nrog denatured antigens yog cov sib xyaw ntawm cov tshuaj tiv thaiv tshwj xeeb rau ib tus neeg epitopes thiab tuaj yeem siv dav dav los kuaj xyuas cov qauv ntuj lossis cov hom phiaj cov protein.Ib qho txiaj ntsig ntawm kev siv cov proteins uas tsis zoo li cov tshuaj tiv thaiv kab mob yog tias cov proteins uas muaj denatured yuav ua rau muaj kev tiv thaiv ntau dua thiab tuaj yeem txhawb kev tiv thaiv kab mob hauv cov tsiaj.
Txoj kev qhia ntawm Escherichia coli feem ntau yog xaiv rau cov hom phiaj antigenic vim tias nws yog cov kab ke kim tshaj plaws ntawm lub sijhawm thiab nyiaj txiag.Txhawm rau txhim kho qhov ua tau ntawm lub hom phiaj qhia protein thiab qhov yooj yim ntawm kev ua kom huv, qee zaum tsuas yog ib feem me me ntawm lub hom phiaj protein, xws li ib qho tshwj xeeb, tau hais.
Peb-dimensional qauv ntawm cov proteins
Qhov tshwj xeeb Domain ntawm Protein
Yog tias lub hom phiaj ntawm kev npaj tshuaj tiv thaiv kab mob tsuas yog rau kev tshawb nrhiav wb xwb, nws yog kev lag luam thiab nrawm siv cov khoom siv me me peptide ua antigen, tab sis muaj kev pheej hmoo ntawm kev tsis muaj zog immunogenicity lossis tsis yog regenicity vim yog kev xaiv tsis tsim nyog ntawm peptide ntu.Txij li kev npaj tshuaj tiv thaiv kab mob yuav tsum tau siv sijhawm ntev, ob lossis peb ntu peptide sib txawv feem ntau raug xaiv los npaj cov tshuaj tiv thaiv uas siv cov tshuaj tiv thaiv polypeptide los xyuas kom meej qhov ua tiav ntawm qhov kev sim.
Lub purity ntawm polypeptide antigen rau kev txhaj tshuaj yuav tsum muaj ntau tshaj 80%.Txawm hais tias qhov purity siab dua tuaj yeem tau txais cov tshuaj tiv thaiv nrog qhov tshwj xeeb zoo dua, hauv kev xyaum, cov tsiaj ib txwm tsim ntau cov tshuaj tiv thaiv kab mob tshwj xeeb, yog li npog cov txiaj ntsig ntawm antigen purity.
Tsis tas li ntawd, kev npaj cov tshuaj tiv thaiv los ntawm cov peptides me me yuav tsum tau txuas mus rau cov kab mob uas tsim nyog los txhim kho nws cov kev tiv thaiv kab mob.Ob hom kab mob antigenic yog KLH thiab BSA.
Post lub sij hawm: Mar-23-2023