Hauv kev tshawb fawb txog roj ntsha, polypeptides nrog cov kab ke ntev feem ntau yog siv.Rau peptides nrog ntau tshaj 60 amino acids nyob rau hauv ib theem zuj zus, gene qhia thiab SDS-PAGE feem ntau yog siv kom tau lawv.Txawm li cas los xij, txoj kev no yuav siv sij hawm ntev thiab qhov kawg ntawm cov khoom sib cais tsis zoo.
Kev sib tw thiab kev daws teeb meem rau kev sib txuas ntawm peptide ntev
Nyob rau hauv lub synthesis ntawm peptides ntev, peb yeej ib txwm ntsib nrog ib tug teeb meem, uas yog, steric hindrance ntawm condensation cov tshuaj tiv thaiv tsub kom nce nrog rau qhov nce nyob rau hauv lub synthesis, thiab lub sij hawm cov tshuaj tiv thaiv yuav tsum tau hloov kho kom cov tshuaj tiv thaiv tiav.Txawm li cas los xij, lub sijhawm ntev dua cov tshuaj tiv thaiv, qhov tshwm sim ntau dua yog tsim, thiab ib feem ntawm lub hom phiaj peptide yog tsim.Xws li residue - tsis muaj peptide chains yog cov impurities tseem ceeb uas ua rau ntev peptide synthesis.Yog li ntawd, nyob rau hauv kev sib txuas ntawm peptide ntev, qhov teeb meem tseem ceeb uas peb yuav tsum tau kov yeej yog tshawb nrhiav cov tshuaj tiv thaiv zoo thiab cov txheej txheem tshuaj tiv thaiv, thiaj li ua kom cov amino acid condensation cov tshuaj tiv thaiv ntau dua thiab tag nrho.Tsis tas li ntawd, txo lub sij hawm cov tshuaj tiv thaiv, vim hais tias ntev dua lub sij hawm cov tshuaj tiv thaiv, qhov ntau uncontrollable sab kev tshwm sim, qhov complex cov khoom los ntawm cov khoom.Yog li ntawd, peb cov ntsiab lus hauv qab no tau sau tseg:
Microwave synthesis tuaj yeem siv: Rau qee cov amino acids ntsib hauv cov txheej txheem synthesis uas tsis yooj yim rau kev sib xyaw, microwave synthesis tuaj yeem siv.Txoj kev no muaj cov txiaj ntsig zoo kawg nkaus, thiab txo qis lub sijhawm cov tshuaj tiv thaiv, thiab txo qhov tsim ntawm ob qhov tseem ceeb los ntawm cov khoom lag luam.
Fragment synthesis txoj kev yuav siv tau: Thaum qee cov peptides nyuaj los ua ke los ntawm cov txheej txheem synthesis thiab tsis yooj yim rau kev ua kom huv, peb tuaj yeem txais tag nrho cov condensation ntawm ntau cov amino acids hauv ib ntu ntawm peptide mus rau peptide saw tag nrho.Txoj kev no tseem tuaj yeem daws tau ntau yam teeb meem hauv kev sib txuas.
Acylhydrazide synthesis tuaj yeem siv tau: acylhydrazide synthesis ntawm peptides yog ib txoj kev ntawm cov khoom-theem synthesis ntawm N-terminal Cys peptide thiab C-terminal polypeptide hydrazide tshuaj xaiv cov tshuaj tiv thaiv ntawm kev tsim ntawm amide bonds kom ua tiav peptide bonding txoj kev.Raws li txoj hauj lwm ntawm Cys nyob rau hauv cov saw peptide, txoj kev no faib tag nrho cov peptide saw mus rau ntau qhov sib lawv liag thiab sib txuas lawv raws.Thaum kawg, lub hom phiaj peptide tau txais los ntawm cov kua-theem condensation cov tshuaj tiv thaiv.Txoj kev no tsis tsuas yog txo qis lub sijhawm sib txuas ntawm peptide ntev, tab sis kuj tseem ua rau kom cov purity ntawm cov khoom kawg.
Ntev peptide purification
Qhov tshwj xeeb ntawm ntev peptides inevitably ua rau complex Cheebtsam ntawm crude peptides.Yog li ntawd, nws kuj yog ib qho nyuaj rau purify ntev peptides los ntawm HPLC.amyloid series ntawm polypeptide purification txheej txheem, absorbing ntau yam kev paub thiab ntse siv nyob rau hauv lub purification ntawm ntev peptide.Los ntawm kev siv cov cuab yeej tshiab, kev sib xyaw ntawm ntau lub tshuab ua kom huv, rov sib cais thiab lwm yam kev paub dhau los, qhov ua tiav ntawm kev ua kom huv peptide ntev tau txhim kho zoo heev.
Lub sij hawm xa tuaj: Plaub Hlis-18-2023