Thymus txoj kev tshiab yog tsim los ntawm 28 amino acid hluavtaws polypeptide tiv thaiv kab mob.Thymus txoj kev yog ib hom tshuaj tshiab uct, feem ntau siv rau cov neeg mob qog, tuaj yeem txhim kho kev tiv thaiv.
Mechanism ntawm kev ua
Qhov tseeb mechanism ntawm thymalfasin nyob rau hauv kev kho mob ntawm tus kab mob siab B tsis tau kawm tag nrho, tab sis ntau yam kev sim tau pom tias thymalfasin muaj lub cev tiv thaiv kab mob tseem ceeb.Nws yog tsuas yog ua tiav los ntawm kev txhim kho T cell muaj nuj nqi, thiab nws khiav los ntawm cov xeev uas koom nrog T cell ntau lawm, faib thiab kho kom zoo, thiab apoptosis.Los ntawm kev nce tus naj npawb ntawm lymphoid, intrahepatic natural killer, CD3 thiab CD4 hlwb, thymalfasin tuaj yeem ua lub luag haujlwm immunomodulatory.Pleural fasin tuaj yeem txhawb kev loj hlob thiab kev faib tawm ntawm DC hlwb, thiab yog li txhawb kev loj hlob thiab faib ntawm T hlwb.Thymalfasin tuaj yeem qhib mitogen lossis antigen, txhim kho kev tsim ntau yam cytokines xws li interferons human interleukin IL2 thiab IL3, txhim khu kev qhia ntawm interleukin-2 receptor, thiab ua rau CD4T hlwb sib txawv rau hauv THLS, uas muaj cov tshuaj tiv thaiv kab mob tseem ceeb.Los ntawm kev nce lub luag haujlwm ntawm P hlwb, nws tuaj yeem txhawb kev tsim cov lymphocytes hauv vivo, kho qhov kev faib ua feem ntawm P cell subsets thiab U cell sib txawv, nrog rau kev tsim cov tshuaj tiv thaiv kab mob, txhawb kev tsim tawm ntawm lwm cov tshuaj tua kab mob lymphokines thiab macrophages, txhim khu kev nqhis dej. ua haujlwm, thiab txhim kho kev ua haujlwm ntawm cellular tiv thaiv kab mob hauv cov neeg mob qog noj ntshav.Nws tseem tuaj yeem tshem tawm cov kab mob, kab mob thiab kab mob ntawm lub cev.Thymus txoj kev phau ntawv rau lub paj hlwb thiab ib puag ncig endothelium, sawv los kho lub luag hauj lwm ntawm hippocampal neuron synaptic kis tau tus mob.Thymus txoj kev tshiab excitatory synaptic kis tau tus mob thiab lub paj hlwb, tuaj yeem siv los tiv thaiv cov neeg mob qog noj ntshav los ntawm kev kho mob qog noj ntshav xws li kws khomob thiab neurotoxicity.Pleuraxin muaj cov tshuaj tswj cov noob caj noob ces, tuaj yeem ua rau kinase cascade noob, nce cytokine noob, tiv thaiv kab mob kev tswj hwm noob.Kev tswj hwm ntawm thymalfasin ntawm IL-zoo li receptor noob qhia tias thymalfasin txhawb nqa cov tshuaj tiv thaiv kab mob."Tsis tas li ntawd, cov txiaj ntsig ntawm thymalfasin ntawm kev loj hlob ntawm lub cev tiv thaiv kab mob yog cuam tshuam los ntawm kev nthuav tawm ntau ntxiv ntawm cov qia cell loj hlob thiab hematopoietic protein noob."
Cov kab ke tshiab ntawm thymectomy
Txheej txheem ntawm kev npaj
1. Biochemical extraction method Thymosin npaj (ib qho sib xyaw ntawm peptides) yog qhov pib tau los ntawm cov ntaub so ntswg tsiaj, yog li kev ua haujlwm txawv heev.Vim tias nws twb muaj cov tsiaj protein, muaj feem ntau dua cov tsos mob ua xua.Cov txheej txheem biochemically tau los ntawm thymus los ntawm tib neeg embryonic thymus yog tshiab, nrog tsawg tawm los tab sis zoo heev.Xav txog cov khoom siv tsawg tsawg, tus nqi siab, tsis tshua muaj txiaj ntsig, thiab cov txheej txheem kev yuav khoom nyuaj., loj-scale tsim teeb meem, kev loj hlob prospect tsis optimistic.
2. Tshuaj synthesis Commercially muaj thymusfasin yog tsim los ua SPSS, uas yog generated thiab purified los ntawm cov tswv yim tsim nyog.Raws li cov resin, Fmoc tau siv rau cov theem tsim khoom, thiab 28 hom amino acids tau tsim raws li thymalfasin tig.N-terminal amino acids tau acetylated nrog acetic anhydride, thiab TFA tau siv los ua ib qho tseem ceeb ntawm kev tawg reagent rau kev tawg kom tau cov khoom ntxhib.Cov khoom nyoos raug cais los ntawm HPLC kom tau txais cov khoom siv thymus tshiab, uas yog tom qab ntawd ua kom huv ntxiv kom tau txais cov khoom siv thymus tshiab.
Muaj peb hom kab mob genetic engineered
1) Ntau cov noob qoob loo yog cloned rau hauv tus tswv tsev kom tau txais cov noob hom phiaj, thiab thaum kawg tau nthuav tawm hauv daim ntawv ntawm kev suav nrog lub cev.Nws tau raug tshaj tawm tias thymmethod tshiab ntau txoj hlua noob tau tsim los ntawm random annealing thiab PCR cov txheej txheem thiab rov ua dua rau hauv Escherichia coli.(2) Cov noob qoob loo uas tsim tawm tau rov ua dua rau hauv cov xov xwm, thiab cov xov xwm tau hloov mus rau hauv tus tswv tsev los tsim cov kab mob genetic engineered.Piv txwv li, raws li lub teeb liab peptide noob nyob rau hauv lub plasmid, cov lus qhia plasmid tau tsim, hloov mus rau hauv Pichia pastoris los ntawm electroexcitation, thiab ces cov fusion protein yog induced los ntawm methanol, thiab ces tus tshiab thymus txoj kev yog tsim los ntawm enzyme digestion thiab lwm yam txhais tau tias. .
Post lub sij hawm: Sep-08-2023