Kev hloov kho methylation

Methylation-hloov peptides, tseem hu ua methylation-paub peptides, yog cov protein tom qab kev txhais lus kho kom zoo nkauj (PTMs) thiab ua lub luag haujlwm tseem ceeb hauv yuav luag txhua lub neej ua haujlwm hauv hlwb.Proteins yog catalyzed los ntawm methyltransferase kom hloov hydroxyl pawg rau cov amino acid residues rau covalent khi.Methylation yog cov txheej txheem hloov pauv hloov pauv tau catalyzed los ntawm demethylase.

Cov kev tshawb fawb tau pom tias cov methylated / demethylated amino acids feem ntau yog lysine (Lys) thiab arginine (Arg).Cov kev tshawb fawb tau pom tias histone lysine methylation muaj ntau yam kev ua haujlwm lom neeg xws li qia cell tu thiab faib, X chromosome inactivation, transcription regulation thiab DNA puas teb.", feem ntau cuam tshuam rau chromatin condensation thiab represses gene qhia."Histone arginine methylation plays lub luag haujlwm tseem ceeb hauv kev tswj hwm ntawm noob caj noob ces thiab tuaj yeem cuam tshuam ntau yam txheej txheem physiological hauv hlwb, suav nrog DNA kho, teeb liab hloov, kev loj hlob ntawm tes, thiab carcinogenesis.Yog li ntawd, Guopeptide Biology tau tsim tshwj xeeb hauv kev tsim cov thev naus laus zis ntawm methyl decorative peptides, uas tau hloov kho los ntawm cov kws tshawb fawb tom qab kev txhais lus protein (PTMS) los pab hauv kev tshawb fawb.

Kev hloov kho Methylation (Me1, Me2, Me3)

Zoo Fmoc-Lys(Me, Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me, Pbf)-OH, Fmoc-Arg(Me) 2-OH.HCl (asymmetrical), F tau siv moc-Arg (kuv) 2-OH.HCl (symmetrical) thiab lwm yam ntaub ntawv raw tau tsim los ntawm FMOC cov txheej txheem sib xyaw ua ke kom tau Lys thiab Arg methylated peptides, thiab cov khoom lag luam tau purified los ntawm HPLC.Cov huab hwm coj txheeb ze, HPLC chromatograms thiab COA yog muab rau cov khoom tiav.