Integrin, los yog integrin, yog heterodimer transmembrane glycoprotein receptor uas kho cov tsiaj cell adhesion thiab signaling.Nws yog tsim los ntawmα thiabβ subunits.Nws koom nrog hauv kev ua kom zoo ntawm ntau yam ntawm tes ua suav nrog kev txav chaw ntawm tes, cell infiltration, cell thiab intercellular signaling, cell adhesion, thiab cov txheej txheem angiogenesis.Integrinαvβ3 tam sim no tau tshawb nrhiav ntau dua.Cov tsos mob ntawm integrinαvβ3 muaj feem xyuam rau cov qog migration, angiogenesis, o thiab osteoporosis.Integrin yog heev nthuav tawm nyob rau hauv tag nrho cov qog nqaij hlav thiab endothelial cell membranes ntawm neovascularization.Cov tsos mob ntawm integrin yog ze ze rau cov qog migration thiab angiogenesis.Nyob rau hauv xyoo tas los no, kev tshawb fawb tshawb fawb tau pom tias muaj 11 integrins uas tuaj yeem khi tshwj xeeb rau RGD peptide, uas yog antagonistic peptides rau integrin receptors.
RGD peptide yog cais rau hauv linear RGD peptide thiab RGD cyclic peptide.Piv nrog rau linear RGD peptide, RGD cyclic peptide muaj zog receptor compatibility thiab receptor tshwj xeeb.Cov hauv qab no yog cov hom thiab kev sib txuas ntawm RGD cyclic peptide.
Hom hom RGD cyclic peptides:
1. Cyclic peptides uas muaj RGD sequences tsim los ntawm disulfide bonds
2. Cyclic peptides uas muaj RGD sequences tsim los ntawm amide bonds
Synthesis ntawm RGD cyclic peptide:
Cov qauv siv hluav taws xob cuam tshuam nrog RGD cyclic peptide synthesis txheej txheem hauv thaj chaw ntawm cov khoom siv theem polypeptide synthesis.Txoj kev tshiab yog xaiv 2-chloro-triphenylmethyl chloride resin raws li tus neeg nqa khoom yuav tsum tau ua ua ntej, ua ntej txuas thawj sab saw carboxyl pawg nrog cov pab pawg tshwj xeeb tiv thaiv D aspartic acid amino acid, tom qab ntawd txuas cov kab peptide ntawm RGD kab ke peptide rau cov cob. , thiab kawg amino acid tshem tawm cov pab pawg tiv thaiv FMOC yam tsis muaj piperidine.Cov txheej txheem catalyst tau ntxiv kom tshem tawm sab saw carboxyl tiv thaiv pawg thawj D aspartic acid ncaj qha los ntawm cov cob, tom qab ntawd los ntawm kev sib ntxiv ntawm piperidine kom tshem tawm cov amino tiv thaiv pawg FMOC ntawm qhov kawg amino acid, tom qab ntawd los ntawm kev sib ntxiv ntawm kev sib txuas tus neeg sawv cev. kom lub cev qhuav dej thiab condense cov carboxyl pawg thiab cov amino pawg tawm ntawm lub taub hau thiab qhov kawg ntawm cov kab peptide ncaj qha los ntawm cov resin nyob rau hauv daim ntawv ntawm amide daim ntawv cog lus los tsim cyclic peptide.Thaum kawg, cyclic peptide raug txiav ncaj qha los ntawm cov cob nrog cov tshuaj txiav.
Post lub sij hawm: Apr-24-2023