Raws li txoj kev sib txuas ntawm amino acid thiab qab zib, qab zib peptide tuaj yeem muab faib ua plaub pawg: O glycosylation, C a N glycosylation, dew saccharification thiab GPI (glycophosphatidlyinositol) kev sib txuas.
1. N-glycosylation glycopeptides yog tsim los ntawm N-acetamide qabzib ntawm qhov txo qhov kawg ntawm glycan saw (Glc-Nac) txuas rau N atom ntawm pawg amide ntawm sab saw ntawm qee cov Asn hauv cov saw peptide, thiab Asn muaj peev xwm txuas cov glycan saw yuav tsum nyob rau hauv AsN-X-Ser / Thr (X! = P) nyob rau hauv lub motif tsim los ntawm cov residues.Cov piam thaj yog N-acetylglucosamine.
N-glycosylation hloov kho cov qauv glycopeptide
2. Cov qauv ntawm O-glycosylation yog yooj yim dua N-glycosylation.Qhov no glycopeptide feem ntau luv dua li glycan, tab sis muaj ntau hom N-glycosylation.Ser thiab Thr feem ntau tuaj yeem ua glycosylated hauv peptide saw.Tsis tas li ntawd, muaj glycopeptides dai kom zoo nkauj nrog tyrosine, hydroxyl, thiab hydroxyproline glycosylation.Txoj hauj lwm txuas yog hydroxyl oxygen atom ntawm sab saw ntawm cov seem.Cov suab thaj sib txuas yog galactose lossis N-acetylgalactosamine (Gal&GalNAc) lossis qabzib / glucosamine (Glc / GlcNAc), mannose / mannosamine (Txiv neej / ManNAc), thiab lwm yam.
O-glycosylation hloov cov qauv
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
Ib qho N-acetylglucosamine (GlcNAc) glycosylation txuas cov proteins O-GlcNAc rau hydroxyl oxygen atom ntawm serine lossis threonine residue ntawm cov protein.O-GlcNA glycosylation yog O-GlcNAc monosaccharide ornament yam tsis muaj glycan extension;Zoo li peptide phosphorylation, O-GlcNAc glycosylation ntawm glycopeptides kuj yog cov txheej txheem kho kom zoo nkauj protein ntau.Kev kho kom zoo O-GlcNAc txawv txav tuaj yeem ua rau muaj ntau yam kab mob xws li ntshav qab zib, kab mob plawv, qog, Alzheimer's kab mob thiab lwm yam.
Glycosylation cov ntsiab lus ntawm glycopeptides
Cov txheej txheem yooj yim ntawm polypeptide thiab qab zib chains yog txuas rau cov saw hlau protein los ntawm covalent bonds, thiab cov chaw txuas cov khoom qab zib yog hu ua glycosylation sites.Txij li thaum tsis muaj cov qauv ua raws li biosynthesis ntawm glycopeptide qab zib chains, cov suab thaj sib txawv yuav raug txuas mus rau tib qhov chaw glycosylation, ua rau lub npe hu ua microscopic inhomogeneity.
Glycosylation ntawm glycopeptides
1. Cov nyhuv ntawm glycopeptide glycosylation ntawm kev kho mob-kev ua tau zoo ntawm kev kho cov proteins
Nyob rau hauv cov ntaub ntawv ntawm kev kho-kho cov proteins, glycosylation kuj cuam tshuam rau ib nrab-lub neej thiab lub hom phiaj ntawm cov tshuaj protein hauv vivo.
2. Soluble glycopeptide glycosylation thiab proteins
Cov kev tshawb fawb tau pom tias cov piam thaj chains ntawm cov proteins tuaj yeem txhim kho cov molecular solubility ntawm cov protein
3. Glycopeptide glycosylation thiab protein immunogenicity
Ntawm qhov tod tes, cov piam thaj chains nyob rau saum npoo ntawm cov proteins tuaj yeem ua rau lub cev tiv thaiv kab mob.Ntawm qhov tod tes, cov khoom qab zib tuaj yeem npog qee qhov chaw ntawm cov protein thiab txo nws txoj kev tiv thaiv kab mob
4. Glycopeptide glycosylation uas ua rau kom muaj protein ntau ruaj khov
Glycosylation tuaj yeem ua rau muaj kev ruaj ntseg ntawm cov protein rau ntau yam kev mob denaturation (xws li denaturants, tshav kub, thiab lwm yam) thiab zam kev sib sau ntawm cov proteins.Nyob rau tib lub sij hawm, cov piam thaj chains nyob rau saum npoo ntawm cov proteins kuj tseem tuaj yeem npog qee qhov proteolytic degradation cov ntsiab lus ntawm cov protein molecules, yog li ua rau muaj kev tiv thaiv ntawm cov proteins rau cov proteinases.
5. Glycopeptide glycosylation uas cuam tshuam rau kev ua haujlwm lom neeg ntawm cov protein molecules
Hloov cov protein glycosylation tuaj yeem ua kom cov protein molecules los tsim cov haujlwm lom neeg tshiab
Post lub sij hawm: Aug-03-2023