Peptide-zoo li synthesis technology
Kev tshawb fawb thiab kev tsim cov tshuaj peptide yog loj hlob sai hauv cov tshuaj.Txawm li cas los xij, kev tsim cov tshuaj peptide tsuas yog txwv los ntawm lawv tus kheej yam ntxwv.Piv txwv li, vim qhov tshwj xeeb rhiab heev rau enzymatic hydrolysis, lub stability yog txo, thiab cov variability ntawm steric conformation ua rau tsis tshua muaj hom phiaj, tsis tshua muaj hydrophobicity, thiab tsis muaj ib tug tshwj xeeb thauj system.Yuav kom kov yeej cov peptides no, tau npaj ntau txoj kev daws teeb meem thiab kev ua tiav ntawm ib hom peptide yog ib qho ntawm lawv.
Hom peptide (Lus Askiv lub npe: Peptoid) lossis Poly - N - tsis yog glycine (Lus Askiv lub npe: Poly real - N - hloov glycine), nws yog quasi peptide compounds ntawm peptide hauv cov saw tseem ceeb.Cov alpha carbon sab saw hloov cov saw hlau loj nitrogen hloov ntawm cov saw hlau sab.Nyob rau hauv thawj polypeptide, pawg R ntawm cov amino acid sab saw sawv cev rau 20 txawv amino acids, tab sis pawg R muaj ntau txoj kev xaiv nyob rau hauv peptoid.Hauv peptide, peptide ntawm cov saw tseem ceeb ntawm cov amino acids nyob rau hauv alpha carbon nitrogen es tsis txhob sab saw hloov mus rau cov saw tseem ceeb.Nws yog ib qho tsim nyog hais tias peptides feem ntau tsis tsim cov txheej txheem qib siab zoo ib yam li cov txheej txheem thib ob hauv peptides thiab cov proteins vim tsis muaj hydrogen ntawm cov pob txha nitrogen.Peptide thawj lub hom phiaj yog tsim kom ruaj khov thiab protease peptide ntawm cov tshuaj me me.
Kev tshuaj xyuas cov txheej txheem zoo li peptide synthesis
Cov txheej txheem ntawm peptide synthesis tau qhia
Txoj kev sib txuas zoo li peptide feem ntau yog subsingle synthesis txoj kev tsim los ntawm RonZuckermann, txhua tus tau muab faib ua ob kauj ruam: acylation thiab hloov chaw.Hauv acylation, thawj kauj ruam yog qhib lub haloacetic acid kom hnov mob nrog cov amines uas tseem tshuav ntawm qhov kawg ntawm cov kauj ruam dhau los, feem ntau yog diisopropyl carbonized diimine.bromoaceticacid tau qhib los ntawm diisopropylcarbodiimide."Nyob rau hauv kev hloov pauv hloov pauv (kev hloov pauv bimolecular nucleophilic cov tshuaj tiv thaiv), ib qho amine, feem ntau yog thawj, tawm tsam lwm yam halogen los ua ib qho N-hloov glycine."Txoj kev subunitary hluavtaws siv tau yooj yim muaj thawj amines los tsim peptides, yog li ua kom cov tshuaj synthesis ntawm peptides.
Khoom txuas ntxiv hauv chav kawm peptides synthesis muaj kev paub nplua nuj, tuaj yeem muab koj ntau yam kev pabcuam peptide synthesis.
Kev tshuaj xyuas cov txheej txheem zoo li peptide synthesis
Qhov zoo ntawm xws li peptide
Ntau ruaj khov: peptoids ruaj khov hauv vivo dua li peptides.
Kev xaiv ntau dua: Peptoids zoo haum rau kev tshawb nrhiav tshuaj sib xyaw ua ke vim tias muaj ntau yam sib txawv ntawm polypeptide tsev blocks tuaj yeem tau txais los ntawm kev hloov kho cov pob txha amino pawg.
Kev ua tau zoo dua: Kev nplua nuj ntawm cov qauv peptoid tuaj yeem ua rau peptoid qhov kev xaiv zoo rau kev tshuaj ntsuam xyuas kom sai sai nrhiav cov qauv tshwj xeeb uas khi rau cov protein.
Ntau lub lag luam muaj peev xwm: cov yam ntxwv ntawm hom peptide cia nws ua ib hom tshuaj txhim kho muaj peev xwm zoo.
Post lub sij hawm: Dec-07-2023